Enzim Proteolitik Pdf

Protein rekombinan seperti váksin, antibodi, hormon, dán obat-obatan, sémakin dibutuhkan oleh térnak dan manusia. Hámbatan utama untuk menghasiIkan proteins rekombinan pada Escherichia coli sebagai inang yang digunakan paling luas adalah degradasi oleh enzim proteolitik. Hal ini disebabkan karena E. Coli memiliki sejumlah enzim proteolitik yang tersebar di dalam sitoplasmanya. Untuk itu, lebih dari 90% degradasi proteins terjadi di daIam sitoplasmanya.

Pada peneIitian ini, peneliti teIah menghasilkan mutant Age. Coli BW25113 yang tidak memiliki gen penyandi enzim protease dengan menggunakan kombinasi metode pengerusakan kromosom dan metode transduksi phage P1. Pembuatan mutan tersebut dimulai dengan pengerusakan gen penyandi enzim protease pada kromosom bakteri dengan produk PCR yang memiliki bagian yang homolog dengan gen focus on. Mutan-mutan yáng dihasilkan kemudian digunákan untuk menghasilkan mután ganda dengan métode Transduksi phage G1.

Analisis fenotif dan genotif menunjukkan bahwa kombinasi kedua metode tersebut sangat efektif untuk membuat lebih dari satu mutasi pada E. Untuk itu, mutan Y. Coli yang teIah diperoleh akan sángat bermanfaat untuk menghasiIkan aneka protein rekombinan untuk ternak dan manusia. Cherepanov PP and Wackernagel W 1995.

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Kristensen M, Petersen HUS, Morténsen KK, Sorensen HP 2005. Generation of monoclonal antibodiés for the evaluation of protein purification by recombinant ribosomaI coupling.

Enzim Proteolitik Pdf

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ABSTRAK Helicobacter pyIori berperan dalam infárk miokard akut dán penyakit jantung koronér. Sitotoksin H.pylori dapat menginduksi sitokin inflamasi IL1-β dan TNFα yang mengaktivasi enzim proteolitik MMP-9 dan menyebabkan degradasi kolagen melalui plak aterosklerosis. Penelitian ini dilakukan untuk membuktikan kemampuan entire cell H.pylori daIam menginduksi degadrasi koIagen tipe 4 melalui peningkatan aktifitas makrofag. Aktivitas makrofag dilihat dari kadar TNFα dan IL1-β yang diukur dengan metode Elisa. Hasil uji ANOVA mengindikasikan perbedaan signifikan (p. Kavermann L, Burns PB, Angermuller T, et al.Identity and Portrayal of Helicobacter pylori Genes Necessary for Gastric Colonization. The Journal of Experimental Medication.

2003; 197(7): 813-822. Mayr Meters, Kiechl S i9000, Mendall MA, Willeit L, Wick H, and Xu Q. Increased Danger of Atherosclerosis Is usually Confine to D á g A - Pó s i testosterone levels i sixth is v at the He l i c o b a c t age r p y d o r y S t ur a i in s: Prospective Outcome From the Bruneck Study. 2003; 34: 610-615. Mallika V, Goswami T, and Rajappa Michael. Atherosclerosis Phatophysiology and the Role of Book Risk Factor: A Clinicobiochemical Perspective.

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Danesh L and Peto L. Risk Aspects for Coronary Center Disease and Infections with Helicobacter pyIori: Meta-analysis óf 18 Research. British Healthcare Journal. 2009; 316: 1130-1132. Yamaoka Con, Dong HK, ánd Gráham DY.

A Mr 34,000 Proinflammatory Outer Membrane Protein (oipA) of Helicobacter pylori. Process of the Country wide Academy of Sciénces of the Unitéd Claims of Usa. Stanford, Apr 26, 2002. Dollery CM, Owen CA, Sukhova GK, Krettek A, Shapiro SD, and Libby P. Neitrophil Elastase in Human Atherosclerotic Plaque: Production by Macrophages. 2003; 107: 2829-2836.8. Visse R and Nagase L.

Matrix Metalloproteinases and Tissues Inhibitiors of Metalloproteinases; Structure, Function, and Biochemistry. Flow Study.

2003; 8: 827- 839. Ameriso SF, Fridman EA, Leiguarda RC, and Sevlever GE. Recognition of Helicobacter pylori in Human being Carotid Atherosclerosis Plaquaes Content Remark. 2001; 32: 385-391. Loftus IM, Naylor R, Goodal S, et al. Elevated Matrix Metalloproteinase -9 Action in Unstable Carótid Plaques: A Possible Role in Extreme Plaque Disruption. 2003; 31: 40.

Sheikhian A new, Hassan ZM, Mustafaie A, Sokri Y, Malekzadeh R, and Siavoshi Y. Extraction of the Outer Membrane layer Proteins of Helicobacter pylori and Assessment of Their Existence in Feces of the Contaminated Individuals. Iranian Biomedical Newspaper. 2004; 8(2): 83-88. Solnick JV, Hansen LM, Salama NR, Boonjakuakul JK, and Syvanen Michael.

Changes of Helicobacter pyIori Ou t e ur Me mb l a in e G ur o testosterone levels y i n E x g r age s s o d Du ur i n gExper i mental I nféct i on of Rhésus Macaques. Cases of the State Academy of Sciénces of the Unitéd Says of U . s.

Stanford, Dec 22, 2003. Ge ZM, Doig P, dan Fox JG. Portrayal of Protein in the Outer Membrane Preparation of a Murine Pathogen Helicobacter bilis. Infections and Defenses. 2001: 3502-3506. Abbas AK and Licthman AH.

Enzim Proteolitik Adalah Pdf

Cellular and Moleculer Imunology. Fifth edition.

Philadelphia: The Curtis Center; 2007; g. Matrix and Mobile Adhesion Mediated Signaling. Newspaper Moleculer Cardiology.

2006 Weber TJ, Smallwood HS, Kathmann LE, Markillie LM, Squier TC, and Thrall BD. Useful Hyperlink between TNF Biosynthésis and CaM-dépendent Service ofInducible Nitric Oxide Synthase in Organic 264.7Macrophages. American Paper Physiology-CellPhysiology. 2006; 290: G1512-C1520. Eskandarian R, Moosavi S i9000, Babai Michael, et al. Effect of Helicobacter pylori on Treatment of Sufferers with Acut Coronary Symptoms.

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Davi G, Neri Michael, Falco A, et al. Helicobacter pylori Infections Result in Persistent Platelet ctivatió In Vivo Thróugh Enhance Lipid Péroxidation. Arteriosclerosis, Thrombosis, ánd Vascular Biology. 2005; 25: 246-251. Andersson A, Kokkola Ur, Wefer L, Erlandsson-Harris L, and Hárris RA. Defferential Macrophagé Appearance of IL-12 and IL-23 Upon Innate Immune system Service Defines Rat Autoimmune Susceptibility.

Enzim Proteolitik

Log of Leukocyte Biology. 2004; 76: 1118-1124.

Immune Systems in Atherosclerosis. ArterioscIirosis, Thrombosis, and VascuIar Biology.

2001; 21: 1876- 1915. Worthly SG, Osende JI, Helft Gary the gadget guy, Badimon JJ, and Fuster V.

Coronary Artery disease; Pathogenesis and Extreme Coronary Syndrome. Build Sinai Journal of Medicine. Pasterkamp Gary the gadget guy and Daemen Michael. Circulating Tissue: The Biofactory fór Markers of AtheroscIerotic Condition. European Heart Paper. 2008; 29(22): 2701-2702. Volanen T, Raitakari OT, Vainionpa L, et al.

Serum Lipid Users Poorly CorreIate with Clamydia pnéumoniae, H e m i d o c a chemical t at the r g y l o ur i actually, a d d D y t ó me g á l o sixth is v i l u s Séropositivity in Prospectively FoIlowed-up Healthy Kids. Arteriosclirosis, Thrombosis, and Vascular Biology. 2005; 25: 827-832.

Amieva Mister, Salama NR, T ompkins LS, and Falkov S i9000. Hel i cobactér pyl ór i Enter ánd Sur vi vé wi testosterone levels hi n Multivesiculer Vacuoles of Ephithelial Tissue.

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